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Showing posts from September, 2019

CRISPR mediated live imaging of Genome editing and transcription done for the first time.

Crispr, dubbed as the discovery of the century has been used in variety of applications. It is used in genome editing, epigenetic modification, modulation of gene expression, locus identification (CASFISH). Recently it has also been used in drug delivery. Crispr has revolutionised biology.

Traditional fluorescent in situ hybridization (FISH) requires DNA denaturation, while fluorescent labeled-dCas9 assembled in vitro with